Isolation of circulating tumor cell: CTC

• CTC is defined as the metastatic cells which are infiltrated into peripheral blood by BIBseparating from primary tumor tissue or metastasis tumor tissue.

• The monitoring of CTC leads to judgement of incipient curative effect and to
• BIBprognostic prediction.
• CTC detection and their typing reveals a pathological condition of patients by BIBexamining expressed biomarkers or genetic mutation and deletion. Therefore CTC BIBtest presents an optimal medical care according to each patient.
• The MACS with antibodies to epithelial marker is used for identifying CTC from blood BIBcells.
• Several researches have reported that metastatic cancer cells enhance the affinity
• BIBfor galactose/galactosamine-specific lectins, such as SBA or HPA. CTC detection of
• BIBLectin method enables cytogenetic analysis by using FISH or PCR serially.

Citation

• Tumor Cell Surface β1-4-Linked Galactose Binds to Lectin(s) on
• BIBMicrovascular Endothelial Cells and Contributes to Organ Colonization.
• BIBThe Journal of Cell Biology, Volume 111, August 1990 773-781B
• Soybean agglutinin binding as a useful prognostic indicator in stomach cancer. BIBSurgery Today　1997, Volume 27(4), 293-297
• Involvement of Soybean Agglutinin Binding Cells in the Lymphatic Metastasis of BIBthe R3230AC Rat Mammary Adenocarcinoma.
• BIBCancer Research 48, 1451-1455, March 15, 1988
• Binding of the lectin from the Roman snail, Helix pomatia agglutinin (HPA),
• BIBto breast cancer cells is linked with a poor prognosis.
• BIBJ Histochem Cytochem. 1995 Mar;43(3):275-81.
• HPA selectively binds to α-N-acetylgalactosamine residues and type A
• BIBerythrocytes. Histol Histopathol. 2000 Jan;15(1):143-58.

Lectin method will be applicable to detection of CTC.

Spread of cytogenetic test

• Lectin method is a promising technology to precisely isolate erythroblasts including whole fetal genome. Recent technologies for the cytogenetic tests such as Next Generation Sequencer, CGH array Digital PCR, and Split FISH, significantly evolve.
• When the applicability of these technologies to the isolated rare cells is demonstrated, the opportunity of cytogenetic test is extended.

Basic functions of lectin
Prenatal test
Efficacy of lectin
Practicability